高糖诱导山羊骨骼肌卫星细胞成脂分化过程中相关基因表达的变化

doi:10.11843/j.issn.0366-6964.2014.05.005

Abstract

Abstract:

This study aimed to induce skeletal muscle satellite cells (SMSCs) to differentiate into adipocytes by using high concentration glucose，and detect the relative expressions of adipogenic related and adipogenic differentiation related genes，and lay a foundation for futher studying the mechanism of SMSCs adipogenic differentiation.High concentration glucose (25 mmol•L^-1) F12 culture medium was used for inducing，and cells were stained with Oil Red O，qPCR was employed to detect the relative expressions of ACC，FAS，C/EBPα，PPARγ，SREBP-1 and AdipoQ at 0 d and five time points (2，4，6，8 and 10 d) under the condition of high and low concentration glucose.The results showed that lots of lipid droplets were observed in cells induced by high concentration glucose at the 6 d.In high concentration glucose group，the mRNA expressions of ACC and C/EBPα significantly increased at the 2 d (P<0.05) and maintained relative high expression levels at later stages，and the expressions of SREBP-1 and FAS were both significantly up-regulated at the 6 d (P<0.05)，while the expression of PPARγ significantly increased at the 10 d (P<0.05).The expression of AdipoQ significantly ascended at the 2，8 and 10 d (P<0.05).In this study，we found that high concentration glucose up-regulated the expressions of the adipogenic-related genes and promoted the SMSCs differentiating into adipocyte-like cells.

CLC Number:

S827
S813.3

XUE Ke， WANG Lin-jie, CHEN Li， WANG Yi-lin， ZHONG Tao， LI Li， ZHANG Hong-ping. Adipogenic-related Gene Expressions in Goat Skeletal Muscle Satellite Cells Treated with High Concentration Glucose[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 706-713.

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Adipogenic-related Gene Expressions in Goat Skeletal Muscle Satellite Cells Treated with High Concentration Glucose

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